1o antibody Search Results


colgalt1 antibody / collagen beta(1-o)galactosyltransferase 1  (NSJ Bioreagents)
N/A
COLGALT1 antibody detects Collagen beta(1-O)galactosyltransferase 1, an enzyme that catalyzes the transfer of galactose to hydroxylysine residues in collagen. This post-translational modification is essential for proper collagen folding, stability, and secretion. COLGALT1 functions within the
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antibody against pofut1  (Proteintech)
92
Proteintech antibody against pofut1
FIGURE 4 Effect of <t>POFUT1</t> and PLAGL2 knockdown on MUC2 expression in WiDr cells. (A) Comparison of POFUT1 and PLAGL2 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (B) Comparison of MUC2 and KRT20 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (C) Western blot analysis comparing the protein expression of POFUT1, PLAGL2, and MUC2 between control and knocked‐down WiDr cells. β‐actin was analyzed as a loading control. **p < .01; *p < .05.
Antibody Against Pofut1, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/POFUT1+Antibody/pm38500386-57-2-6
Average 92 stars, based on 1 article reviews
antibody against pofut1 - by Bioz Stars, 2026-10
92/100 stars
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anti pla2g15  (Proteintech)
92
Proteintech anti pla2g15
FIGURE 4 Effect of <t>POFUT1</t> and PLAGL2 knockdown on MUC2 expression in WiDr cells. (A) Comparison of POFUT1 and PLAGL2 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (B) Comparison of MUC2 and KRT20 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (C) Western blot analysis comparing the protein expression of POFUT1, PLAGL2, and MUC2 between control and knocked‐down WiDr cells. β‐actin was analyzed as a loading control. **p < .01; *p < .05.
Anti Pla2g15, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/LYPLA3+Polyclonal+antibody/bio_rxiv__2024__05__31__596474-176-168-169
Average 92 stars, based on 1 article reviews
anti pla2g15 - by Bioz Stars, 2026-10
92/100 stars
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anti c1galt1  (Proteintech)
93
Proteintech anti c1galt1
FIGURE 4 Effect of <t>POFUT1</t> and PLAGL2 knockdown on MUC2 expression in WiDr cells. (A) Comparison of POFUT1 and PLAGL2 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (B) Comparison of MUC2 and KRT20 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (C) Western blot analysis comparing the protein expression of POFUT1, PLAGL2, and MUC2 between control and knocked‐down WiDr cells. β‐actin was analyzed as a loading control. **p < .01; *p < .05.
Anti C1galt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/C1GALT1+Antibody/pm41402741-115-52-53
Average 93 stars, based on 1 article reviews
anti c1galt1 - by Bioz Stars, 2026-10
93/100 stars
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streptavidin antibody rabbit 1 o ab 1:1,000, genescript pab cat. no. a00621  (GenScript corporation)
90
GenScript corporation streptavidin antibody rabbit 1 o ab 1:1,000, genescript pab cat. no. a00621
Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with <t>streptavidin</t> on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).
Streptavidin Antibody Rabbit 1 O Ab 1:1,000, Genescript Pab Cat. No. A00621, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/streptavidin+antibody+rabbit+1+o+Ab+1+1+000++Genescript+pAb+Cat++No++A00621/pmc09271999-66-5-12
Average 90 stars, based on 1 article reviews
streptavidin antibody rabbit 1 o ab 1:1,000, genescript pab cat. no. a00621 - by Bioz Stars, 2026-10
90/100 stars
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polyclonal rabbit anti-human p-selectin 1 o antibody  (Becton Dickinson)
90
Becton Dickinson polyclonal rabbit anti-human p-selectin 1 o antibody
Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with <t>streptavidin</t> on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).
Polyclonal Rabbit Anti Human P Selectin 1 O Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/polyclonal+rabbit+anti+human+p+selectin+1+o+antibody/pmc01850489-173-11-18
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-human p-selectin 1 o antibody - by Bioz Stars, 2026-10
90/100 stars
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rabbit anti human pofut1 polyclonal antibody  (Boster Bio)
92
Boster Bio rabbit anti human pofut1 polyclonal antibody
Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with <t>streptavidin</t> on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).
Rabbit Anti Human Pofut1 Polyclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/Anti-POFUT1+Antibody/pm37432515-17-0-9
Average 92 stars, based on 1 article reviews
rabbit anti human pofut1 polyclonal antibody - by Bioz Stars, 2026-10
92/100 stars
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anti-p2x2 receptor antibody  (Alomone Labs)
94
Alomone Labs anti-p2x2 receptor antibody
Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with <t>streptavidin</t> on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).
Anti P2x2 Receptor Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/Anti-P2X2+Receptor+Antibody/alomone+labs___apr-003
Average 94 stars, based on 1 article reviews
anti-p2x2 receptor antibody - by Bioz Stars, 2026-10
94/100 stars
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dgat1 antibody / diacylglycerol o-acyltransferase 1  (NSJ Bioreagents)
90
NSJ Bioreagents dgat1 antibody / diacylglycerol o-acyltransferase 1
Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with <t>streptavidin</t> on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).
Dgat1 Antibody / Diacylglycerol O Acyltransferase 1, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1o+antibody/DGAT1+Antibody+%2F+Diacylglycerol+O-acyltransferase+1/custom%40rq8914%4019648280
Average 90 stars, based on 1 article reviews
dgat1 antibody / diacylglycerol o-acyltransferase 1 - by Bioz Stars, 2026-10
90/100 stars
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anti-acta2 monoclonal antibody, clone 1.o.6  (Creative BioMart)
N/A
Recognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial).Actins are highly conserved proteins that are involved in various types of cell motility
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anti-cgb monoclonal antibody, clone 1.o.326 + 1.o.327  (Creative BioMart)
N/A
This antibody recognizes and is highly specific for hCG beta.Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C
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anti-ivl monoclonal antibody, clone 1.o.452  (Creative BioMart)
N/A
Recognizes involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF-7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in dog and a
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Image Search Results


FIGURE 4 Effect of POFUT1 and PLAGL2 knockdown on MUC2 expression in WiDr cells. (A) Comparison of POFUT1 and PLAGL2 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (B) Comparison of MUC2 and KRT20 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (C) Western blot analysis comparing the protein expression of POFUT1, PLAGL2, and MUC2 between control and knocked‐down WiDr cells. β‐actin was analyzed as a loading control. **p < .01; *p < .05.

Journal: Cell biochemistry and function

Article Title: POFUT1 and PLAGL2 are characteristic markers of mucinous colorectal cancer associated with MUC2 expression.

doi: 10.1002/cbf.3989

Figure Lengend Snippet: FIGURE 4 Effect of POFUT1 and PLAGL2 knockdown on MUC2 expression in WiDr cells. (A) Comparison of POFUT1 and PLAGL2 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (B) Comparison of MUC2 and KRT20 expression by qRT‐PCR between control and knockdown WiDr cells (n = 3). (C) Western blot analysis comparing the protein expression of POFUT1, PLAGL2, and MUC2 between control and knocked‐down WiDr cells. β‐actin was analyzed as a loading control. **p < .01; *p < .05.

Article Snippet: The primary antibody against POFUT1 (14929‐1‐AP; Proteintech), PLAGL2 (11540‐1‐ AP; Proteintech), MUC2 (27675‐1‐AP; Proteintech), and β‐actin (ab8227; Abcam) were diluted 1:4000, 1:2500, and 1:1000 respectively, and applied overnight at 4°C.

Techniques: Knockdown, Expressing, Comparison, Quantitative RT-PCR, Control, Western Blot

FIGURE 5 Effect of POFUT1 and PLAGL2 knockdown in vivo. (A) Images of the tumors generated from control and knocked down WiDr cells. Scale bar, 5 mm. (B) Comparison of tumor volumes between control and knocked‐down WiDr‐derived tumors (n = 3). (C) Comparison of MUC2 expression by qRT‐PCR between control and knocked down WiDr‐derived tumors (n = 3). (D) Representative images of IHC on MUC2 in control and knocked down WiDr‐derived tumors. Scale bar, 250 μm. *p < .05.

Journal: Cell biochemistry and function

Article Title: POFUT1 and PLAGL2 are characteristic markers of mucinous colorectal cancer associated with MUC2 expression.

doi: 10.1002/cbf.3989

Figure Lengend Snippet: FIGURE 5 Effect of POFUT1 and PLAGL2 knockdown in vivo. (A) Images of the tumors generated from control and knocked down WiDr cells. Scale bar, 5 mm. (B) Comparison of tumor volumes between control and knocked‐down WiDr‐derived tumors (n = 3). (C) Comparison of MUC2 expression by qRT‐PCR between control and knocked down WiDr‐derived tumors (n = 3). (D) Representative images of IHC on MUC2 in control and knocked down WiDr‐derived tumors. Scale bar, 250 μm. *p < .05.

Article Snippet: The primary antibody against POFUT1 (14929‐1‐AP; Proteintech), PLAGL2 (11540‐1‐ AP; Proteintech), MUC2 (27675‐1‐AP; Proteintech), and β‐actin (ab8227; Abcam) were diluted 1:4000, 1:2500, and 1:1000 respectively, and applied overnight at 4°C.

Techniques: Knockdown, In Vivo, Generated, Control, Comparison, Derivative Assay, Expressing, Quantitative RT-PCR

FIGURE 6 Expression of POFUT1 and PLAGL2 in human CRCs. (A) Representative images of IHC on POFUT1, PLAGL2, and MUC2 in human MAC and NMAC tissues. Scale bar, 200 μm (B) Comparison of IHC scores between MAC and NMAC tissues. (n = 15 each). **p < .01; *p < .05. CRC, colorectal cancer; IHC, immunohistochemistry; MAC, mucinous adenocarcinoma; NMAC, nonmucinous adenocarcinoma.

Journal: Cell biochemistry and function

Article Title: POFUT1 and PLAGL2 are characteristic markers of mucinous colorectal cancer associated with MUC2 expression.

doi: 10.1002/cbf.3989

Figure Lengend Snippet: FIGURE 6 Expression of POFUT1 and PLAGL2 in human CRCs. (A) Representative images of IHC on POFUT1, PLAGL2, and MUC2 in human MAC and NMAC tissues. Scale bar, 200 μm (B) Comparison of IHC scores between MAC and NMAC tissues. (n = 15 each). **p < .01; *p < .05. CRC, colorectal cancer; IHC, immunohistochemistry; MAC, mucinous adenocarcinoma; NMAC, nonmucinous adenocarcinoma.

Article Snippet: The primary antibody against POFUT1 (14929‐1‐AP; Proteintech), PLAGL2 (11540‐1‐ AP; Proteintech), MUC2 (27675‐1‐AP; Proteintech), and β‐actin (ab8227; Abcam) were diluted 1:4000, 1:2500, and 1:1000 respectively, and applied overnight at 4°C.

Techniques: Expressing, Comparison, Immunohistochemistry

Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with streptavidin on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).

Journal: Frontiers in Physiology

Article Title: Role of SNARE Proteins in the Insertion of KCa3.1 in the Plasma Membrane of a Polarized Epithelium

doi: 10.3389/fphys.2022.905834

Figure Lengend Snippet: Membrane location of KCa3.1-BLAP stably expressed in FRT cells. The sidedness experiments confirm the expression pattern of KCa3.1 on the basolateral membrane of FRT cells. Untransfected FRT cells or FRT-KCa3.1-BLAP cells were grown on Transwell™ filter inserts to form a monolayer. Untransfected cells were labeled with streptavidin on both sides of the filter; while FRT-KCa3.1-BLAP cells were labeled either on the apical side or the basolateral side of the filter insert. A band was only observed in the sample that was labeled basolaterally near the 82 kD mark. Lane 1: untransfected FRT, Lane 2: KCa3.1-BLAP- + Apical streptavidin labeling, and Lane 3: KCa3.1-BLAP + Basolateral streptavidin labeling. Each lane was loaded with 30 µg of protein and β-actin was used as a loading control ( n = 5).

Article Snippet: Antibody dilutions were as follows streptavidin antibody (rabbit 1 o Ab 1:1,000, Genescript pAb Cat. No. A00621, 2 o donkey anti-rabbit 1:10,000, Amersham Cat. No. NA934, Sigma-Aldrich, New Zealand (NZ)); VAMP3 antibody (rabbit 1 o Ab 1:1,000, Sapphire Bioscience LS- Cat. No. C144617, NZ, 2 o donkey anti-rabbit 1:10,000); SNAP-23 (mouse 1 o Ab 1:1,000, Santa Cruz Biotechnology (A5) Cat. No. SC-166244 Dallas, TX, 2 o goat anti-mouse 1:2000 Sigma-Aldrich Cat. No. A4416, NZ); STX-4 (rabbit 1 o Ab 1:1,000, Merck Cat. No. 574788, Germany, 2 o donkey anti-rabbit 1:10,000); GAPDH (rabbit 1 o Ab 1:1,000, Sigma-Aldrich Cat. No. G9545, NZ); and β-actin IgG antibody (mouse 1:10,000, Sigma-Aldrich Cat. No. A5441, NZ).

Techniques: Membrane, Stable Transfection, Expressing, Labeling, Control

KCa3.1 co-immunoprecipitates (Co-IP) with VAMP3, SNAP-23 and Syntaxin-4 (STX-4). KCa3.1-BLAP in FRT cells was used as bait to determine protein associations with the SNARE proteins VAMP3, SNAP-23 and STX-4. (A) Upper Panel: The blot was probed with anti-SNAP-23 antibody. Lane 1 - SNAP-23 lysate control; Lane 2 - IgG negative control; and Lane 3—pulled down of SNAP-23 ( n = 5). Middle Panel: The blot was stripped and reprobed with anti-VAMP3 antibody. Lane 1—VAMP3 lysate control; Lane 2 - IgG negative control; and Lane 3—VAMP3 pulled down by Co-IP ( n = 6). Lower Panel: The blot was stripped and reprobed with anti-streptavidin antibody. Lane 1—KCa3.1 lysate control; Lane 2—IgG negative control; and Lane 3—KCa3.1 pulled down by Co-IP ( n = 6). (B) Upper Panel: The blot was probed with anti-STX-4 antibody. Lane 1—STX-4 lysate control; Lane 2—IgG negative control; and Lane 3—STX-4 pulled down by Co-IP (band is running high, n = 6). Lower Panel: The blot was stripped and reprobed with anti-streptavidin antibody to detect KCa3.1. The KCa3.1 band is diffuse likely due the membrane being stripped. Lane 1—KCa3.1 lysate control; Lane 2—IgG negative control; Lane 3—KCa3.1 pulled down by Co-IP ( n = 6). These data suggest protein associations occur between KCa3.1 and VAMP3, SNAP-23 and Syntaxin-4.

Journal: Frontiers in Physiology

Article Title: Role of SNARE Proteins in the Insertion of KCa3.1 in the Plasma Membrane of a Polarized Epithelium

doi: 10.3389/fphys.2022.905834

Figure Lengend Snippet: KCa3.1 co-immunoprecipitates (Co-IP) with VAMP3, SNAP-23 and Syntaxin-4 (STX-4). KCa3.1-BLAP in FRT cells was used as bait to determine protein associations with the SNARE proteins VAMP3, SNAP-23 and STX-4. (A) Upper Panel: The blot was probed with anti-SNAP-23 antibody. Lane 1 - SNAP-23 lysate control; Lane 2 - IgG negative control; and Lane 3—pulled down of SNAP-23 ( n = 5). Middle Panel: The blot was stripped and reprobed with anti-VAMP3 antibody. Lane 1—VAMP3 lysate control; Lane 2 - IgG negative control; and Lane 3—VAMP3 pulled down by Co-IP ( n = 6). Lower Panel: The blot was stripped and reprobed with anti-streptavidin antibody. Lane 1—KCa3.1 lysate control; Lane 2—IgG negative control; and Lane 3—KCa3.1 pulled down by Co-IP ( n = 6). (B) Upper Panel: The blot was probed with anti-STX-4 antibody. Lane 1—STX-4 lysate control; Lane 2—IgG negative control; and Lane 3—STX-4 pulled down by Co-IP (band is running high, n = 6). Lower Panel: The blot was stripped and reprobed with anti-streptavidin antibody to detect KCa3.1. The KCa3.1 band is diffuse likely due the membrane being stripped. Lane 1—KCa3.1 lysate control; Lane 2—IgG negative control; Lane 3—KCa3.1 pulled down by Co-IP ( n = 6). These data suggest protein associations occur between KCa3.1 and VAMP3, SNAP-23 and Syntaxin-4.

Article Snippet: Antibody dilutions were as follows streptavidin antibody (rabbit 1 o Ab 1:1,000, Genescript pAb Cat. No. A00621, 2 o donkey anti-rabbit 1:10,000, Amersham Cat. No. NA934, Sigma-Aldrich, New Zealand (NZ)); VAMP3 antibody (rabbit 1 o Ab 1:1,000, Sapphire Bioscience LS- Cat. No. C144617, NZ, 2 o donkey anti-rabbit 1:10,000); SNAP-23 (mouse 1 o Ab 1:1,000, Santa Cruz Biotechnology (A5) Cat. No. SC-166244 Dallas, TX, 2 o goat anti-mouse 1:2000 Sigma-Aldrich Cat. No. A4416, NZ); STX-4 (rabbit 1 o Ab 1:1,000, Merck Cat. No. 574788, Germany, 2 o donkey anti-rabbit 1:10,000); GAPDH (rabbit 1 o Ab 1:1,000, Sigma-Aldrich Cat. No. G9545, NZ); and β-actin IgG antibody (mouse 1:10,000, Sigma-Aldrich Cat. No. A5441, NZ).

Techniques: Co-Immunoprecipitation Assay, Control, Negative Control, Membrane